T-GUIDE
Design guide RNAs for TnpB genome editing across multiple species with various TnpB variants
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| # | Sequence (5'→3') | Position | Strand | GC% | Efficiency* | Off-targets (Mismatches)** | Location (ID) | Actions | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CNN (%) | RNN (%) | MM0 | MM1 | MM2 | MM3 | |||||||
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*Efficiency Models: Calculated using the TEEP TnpB model. Reference: Marquart, K.F., et al. (2024). Effective genome editing with an enhanced ISDra2 TnpB system and deep learning-predicted ωRNAs. Nature Methods.
**Off-target Analysis: Identified using Cas-OFFinder. Reference: Bae, S., et al. (2014). Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics.